Product Pathways - Neuroscience
VAMP2 (D6O1A) Rabbit mAb #13508
|13508S||100 µl (10 western blots)||---||In Stock||---|
|13508||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||13||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
VAMP2 (D6O1A) Rabbit mAb recognizes endogenous levels of total VAMP2 protein. This antibody does not cross-react with VAMP1 protein and recognizes VAMP2 protein by IF-IC analysis in mouse and rat cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human VAMP2 protein.
Western blot analysis of extracts from various cell lines and tissues using VAMP2 (D6O1A) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human VAMP1 (hVAMP1-Myc; +) or VAMP2 (hVAMP2-Myc; +), using VAMP2 (D6O1A) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).
Immunoprecipitation of VAMP2 from Neuro-2a cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or VAMP2 (D6O1A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using VAMP2 (D6O1A) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon using VAMP2 (D6O1A) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded rat kidney using VAMP2 (D6O1A) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse pancreas using VAMP2 (D6O1A) Rabbit mAb.
Confocal immunofluorescent analysis of PC-12 cells using VAMP2 (D6O1A) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Vesicle-associated membrane protein 2 (VAMP2, also called synaptobrevin) is part of the R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex (1). The SNARE complex is involved in vesicular transport and membrane fusion, a process regulated by calcium (2). In neurons, VAMP2 is predominantly inserted in presynaptic vesicle membranes. Assembly of VAMP2 with the plasma membrane SNAREs syntaxin 1 and SNAP25 is a key event necessary for membrane fusion and neurotransmitter release (2). In addition to this important function, VAMP2 is also involved in granule exocytosis in neutrophils (3) and release of bioactive peptides from cardiac myocytes (4) and juxtaglomerular cells (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Tween® is a registered trademark of ICI Americas, Inc.
SignalStain® is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
DyLight™ is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.