Product Pathways - Lymphocyte Signaling
IL-2Rα/CD25 (D6K5F) Rabbit mAb #13517
|13517S||100 µl (10 western blots)||---||In Stock||---|
|13517||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human||Endogenous||35, 60||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
IL-2Rα/CD25 (D6K5F) Rabbit mAb recognizes endogenous levels of total IL-2Rα (CD25) protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala34 of human IL-2Rα (CD25) protein.
Western blot analysis of extracts from HDLM-2, L-540, and PC-3 cell lines using IL-2Rα/CD25 (D6K5F) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from purified human CD4+ and CD8+ T cells using IL-2Rα/CD25 (D6K5F) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human IL-2Rα/CD25 (hIL-2Rα; +), using IL-2Rα/CD25 (D6K5F) Rabbit mAb.
Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2).
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