Product Pathways - Development
TRIB2 (D8P2X) Rabbit mAb #13533
|13533S||100 µl (10 western blots)||---||In Stock||---|
|13533||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||42||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
TRIB2 (D8P2X) Rabbit mAb recognizes endogenous levels of total TRIB2 protein. This antibody does not cross-react with other TRIBBLES family proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to full-length human TRIB2 protein.
Western blot analysis of extracts from various cell lines using TRIB2 (D8P2X) Rabbit mAb.
TRIBBLES proteins belong to a small family of serine-threonine kinase-like proteins characterized by the presence of a variant protein kinase motif (lacking a canonical ATP binding site), a MEK-1 binding site, and a C-terminal COP1 site that binds ubiquitin ligase. The tribbles gene was first identified and characterized in Drosophila genetic screens for genes that regulate cell division, gastrulation and oogenesis (1-3). Research studies in Drosophila suggested that Tribbles functions to coordinate cell division by regulating turnover of the cell cycle protein String/cdc25. In contrast to the Drosophila genome, which contains a single tribbles gene, the genomes of mice and humans encode three known TRIBBLES proteins (TRIB1-3), which exhibit both distinct and overlapping patterns of expression and functions (4). For example, TRIB1 and TRIB2, but not TRIB3, were reported to promote degradation of the basic region-leucine zipper transcription factor C/EBPα, a function that appears to be conserved from flies to humans (5,6). TRIB2 is overexpressed in a subset of human AML patient samples, down-regulated in leukemic cells undergoing proliferation arrest (7), and positively regulated by the NOTCH signaling pathway in T cells (8), while retroviral-mediated overexpression of Trib2 in mice was shown to induce transplantable leukemia (7). These finding collectively suggest that TRIB2 functions as an oncogene in the mammalian hematopoietic system (9).
- Grosshans, J. and Wieschaus, E. (2000) Cell 101, 523-31.
- Seher, T.C. and Leptin, M. (2000) Curr Biol 10, 623-9.
- Mata, J. et al. (2000) Cell 101, 511-22.
- Dobens, L.L. and Bouyain, S. (2012) Dev Dyn 241, 1239-48.
- Dedhia, P.H. et al. (2010) Blood 116, 1321-8.
- Rørth, P. et al. (2000) Mol Cell 6, 23-30.
- Keeshan, K. et al. (2006) Cancer Cell 10, 401-11.
- Hannon, M.M. et al. (2012) Br J Haematol 158, 626-34.
- Liang, K.L. et al. (2013) Blood 121, 4265-70.
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