Product Pathways - Cytoskeletal Signaling
Myosin VI (D5Y5A) Rabbit mAb #13592
|13592S||100 µl (10 western blots)||---||In Stock||---|
|13592||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Dog||Endogenous||150||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Myosin VI (D5Y5A) Rabbit mAb recognizes endogenous levels of total myosin VI protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg1131 of human myosin VI protein.
The myosin family of motor proteins drive ATP-dependent actin-based motility in eukaryotic cells and contain a conserved amino-terminal motor domain (reviewed in 1,2).
Myosin VI is an unconventional minus-end-directed myosin involved in the transport of vesicles and organelles within the cell, endocytosis, and organelle biogenesis (3-6). The movement of myosin VI and its cargo along actin filaments is unique among myosin family members in its mechanism; its tail domain structure allows it to take larger than predicted steps along the actin filament (reviewed in 1,7).
Myosin VI has been shown to regulate the polarized delivery of proteins to specialized subcellular locations, including the delivery of EGFR to the leading edge of migrating cells (8), as well as the delivery of specialized axonal proteins in neurons (9). Myosin VI has also been shown to mediate activity of the tumor suppressor p53 during DNA damage (10,11).
- Sweeney, H.L. and Houdusse, A. (2010) Cell 141, 573-82.
- Nambiar, R. et al. (2010) Cell Mol Life Sci 67, 1239-54.
- Bond, L.M. et al. (2011) Mol Biol Cell 22, 54-65.
- Aschenbrenner, L. et al. (2004) Mol Biol Cell 15, 2253-63.
- Loubéry, S. et al. (2012) Traffic 13, 665-80.
- Ameen, N. and Apodaca, G. (2007) Traffic 8, 998-1006.
- Spudich, J.A. and Sivaramakrishnan, S. (2010) Nat Rev Mol Cell Biol 11, 128-37.
- Chibalina, M.V. et al. (2010) Traffic 11, 1290-303.
- Lewis, T.L. et al. (2011) PLoS Biol 9, e1001021.
- Jung, E.J. et al. (2006) Mol Cell Biol 26, 2175-86.
- Cho, S.J. and Chen, X. (2010) J Biol Chem 285, 27159-66.
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