Product Pathways - PI3K / Akt Signaling
Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb #13619
|13619S||100 µl (10 western blots)||---||In Stock||---|
|13619||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||75||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Phospho-YAP (Ser397) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser397. This residue corresponds to Ser381 of YAP isoform 2, as reported by Zhao, B. et al. (2010) Genes Dev 24, 72-85 (9).
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser397 of human YAP protein isoform 1.
Western blot analysis of extracts from Hep G2 cells, untreated (-) or λ-phosphatase-treated (+), using Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from MDA-MB-231 cells, vehicle treated (-) or treated with epinephrine (10 μM, 60 min; +) or Forskolin #3828 (10 μm, 60 min; +), using Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8547 (lower). YAP protein isoform 1 Ser397 corresponds to Ser381 of YAP isoform 2, as reported by Zhao, B. et al. (2010) Genes Dev 24, 72-85 (9).
Western blot analysis of extracts from various cell lines using Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb.
YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a critical and widely conserved role in regulating tissue growth and organ size. Upon phosphorylation at Ser127, YAP translocates to the cytoplasm, where it is sequestered through association with 14-3-3 proteins in an Akt-dependent manner (6-8).
Phosphorylation of YAP at Ser397 primes YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron. Upon phosphorylation, the phosphodegron recruits the β-TrCP (SCF) ubiquitin ligase complex, which ubiquitinates YAP to trigger its proteolytic degradation in the proteasome (9).
- Sudol, M. (1994) Oncogene 9, 2145-2152.
- Mohler, P. et al. (1999) J. Cell Biol. 147, 879-890.
- Espanel, X. and Sudol, M. (2001) J. Biol. Chem. 276, 14514-14523.
- Sudol, M. et al. (1995) FEBS Lett. 369, 67-71.
- Yagi, R. et al. (1999) EMBO J. 18, 2551-2562.
- Basu, S. et al. (2003) Mol. Cell 11, 11-23.
- Zhao, B. et al. (2010) Genes Dev 24, 862-74.
- Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
- Zhao, B. et al. (2010) Genes Dev 24, 72-85.
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