Product Pathways - Peptides
Phospho-Btk (Tyr223) Biotinylated Peptide #1460
Cell Signaling Technology offers a full line of protein kinases, substrates, antibody detection reagents and HTScan® kits. Browse our "Reagents for High-Throughput Screening" product listing or contact us at drugdiscovery@cellsignal.com.
Description
This biotinylated peptide contains the residues surrounding Tyr223 of Btk. The tyrosine residue in the peptide corresponding to Tyr223 has been chemically phosphorylated in the course of peptide synthesis. This phosphopeptide was generated for use as a positive control in CST's HTScan® EphB3 Kinase Assay Kit #7716, but it may also serve as a positive control in other heterogeneous or homogeneous kinase assays.
Quality Control
The quality of the biotinylated peptide was evaluated by reverse-phase HPLC and by mass spectrometry.
Peptide Core Sequence
VALY*DYM
Molecular Weight
2132 daltons
Directions for Use
The phosphorylated form of the peptide can be detected with the Phospho-Tyrosine mAb (P-Tyr-100) #9411. Sample kinase assay protocol is attached.
Application References
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