Product Pathways - Peptides
Phospho-Autocam Biotinylated Peptide #1511
Cell Signaling Technology offers a full line of protein kinases, substrates, antibody detection reagents and HTScan® kits. Browse our "Reagents for High-Throughput Screening" product listing or contact us at drugdiscovery@cellsignal.com.
Description
Autocam is a synthetic peptide derived from the Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII) autophosphorylation site that serves as a selective CaMKII substrate. The threonine residue in the peptide has been chemically phosphorylated in the course of peptide synthesis. This phosphopeptide was generated for use as a positive control in kinase assays (CaMKI-γ Kinase #7456, MAPKAPK-2 Kinase #7442) but it may also serve as a positive control in other heterogeneous or homogeneous kinase assays.
Quality Control
The quality of the biotinylated peptide was evaluated by reverse-phase HPLC and by mass spectrometry.
Peptide Core Sequence
RQET*VDA
Molecular Weight
2088 daltons
Directions for Use
This phosphorylated peptide can be detected with the Phospho-Threonine (42H4) Mouse mAb #9386. A sample kinase assay protocol is attached.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
