Product Pathways - Apoptosis
BID Antibody (Human Specific) #2002
|2002S||100 µl (10 western blots)||---||In Stock||---|
|2002P||40 µl (4 western blots)||---||In Stock||---|
|2002||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
BID Antibody (Human Specific) detects endogenous levels of both the full length (22 kDa) and cleaved large fragment (15 kDa) of human BID.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of human BID. Antibodies are purified by protein A and peptide affinity chromatography.
Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax (1). Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane (2-4). Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation (5-7). Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation (8,9).
- Wang, K. et al. (1996) Genes Dev 10, 2859-69.
- Luo, X. et al. (1998) Cell 94, 481-90.
- Li, H. et al. (1998) Cell 94, 491-501.
- Gross, A. et al. (1999) J Biol Chem 274, 1156-63.
- Li, P. et al. (1997) Cell 91, 479-89.
- Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
- Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
- Yin, X.M. et al. (1999) Nature 400, 886-91.
- Korsmeyer, S.J. et al. (2000) Cell Death Differ 7, 1166-73.
- Mandic, A. et al. (2002) Mol. Cell. Biol. 22 (9), 3003-3013. Applications: Western Blotting.
- Chandrasekar, B. et al. (2004) J. Biol. Chem. 279, 20221-20233. Applications: Western Blotting.
- Ogino, T. et al. (2009) Leuk Res 33, 151-8. Applications: Western Blotting.
- Son, J.K. et al. (2010) Cell Death Differ 17, 1288-301. Applications: Western Blotting.
- Xiang, X. et al. (2011) PLoS One 6, e14640. Applications: Western Blotting.
- Jane, E.P. et al. (2011) Mol Cancer Ther 10, 198-208. Applications: Western Blotting.
- Chacko, A.D. et al. (2010) BMC Cancer 10, 380. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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