Product Pathways - Apoptosis

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BID Antibody (Human Specific) #2002

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H	Endogenous	15, 22	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

2002:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

BID Antibody (Human Specific) detects endogenous levels of both the full length (22 kDa) and cleaved large fragment (15 kDa) of human BID.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of human BID. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax (1). Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane (2-4). Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation (5-7). Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation (8,9).

1. Wang, K. et al. (1996) Genes Dev 10, 2859-69.
2. Luo, X. et al. (1998) Cell 94, 481-90.
3. Li, H. et al. (1998) Cell 94, 491-501.
4. Gross, A. et al. (1999) J Biol Chem 274, 1156-63.
5. Li, P. et al. (1997) Cell 91, 479-89.
6. Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
7. Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
8. Yin, X.M. et al. (1999) Nature 400, 886-91.
9. Korsmeyer, S.J. et al. (2000) Cell Death Differ 7, 1166-73.

Application References

• Mandic, A. et al. (2002) Calpain-mediated Bid cleavage and calpain-independent Bak modulation: two separate pathways in cisplatin-induced apoptosis. Mol. Cell. Biol. 22 (9), 3003-3013. Applications: Western Blotting
• Chandrasekar, B. et al. (2004) Activation of intrinsic and extrinsic proapoptotic signaling pathways in interleukin-18-mediated human cardiac endothelial cell death. J. Biol. Chem. 279, 20221-20233. Applications: Western Blotting
• Ogino, T. et al. (2009) Leuk Res 33, 151-8. Applications: Western Blotting
• Son, J.K. et al. (2010) Cell Death Differ 17, 1288-301. Applications: Western Blotting
• Xiang, X. et al. (2011) PLoS One 6, e14640. Applications: Western Blotting
• Jane, E.P. et al. (2011) Mol Cancer Ther 10, 198-208. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 2003 BID Antibody (Mouse Specific)
• 2006 BID (7A3) Mouse mAb (Human Specific)
• 9748 Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb
• 9746 Caspase-8 (1C12) Mouse mAb
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

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For Research Use Only. Not For Use In Diagnostic Procedures.