Product Pathways - Apoptosis / Autophagy
BID Antibody (Mouse Specific) #2003
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | M | Endogenous | 22 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
M=Mouse
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
BID Antibody (Mouse Specific) detects endogenous levels of full length mouse BID protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding the cleavage site of mouse BID. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from L929 cells, untreated, etoposide-treated (25 µM) or TNF-α-treated (20 ng/ml), using BID Antibody (Mouse Specific).
Background
The BH3 domain-only protein, BID, a death agonist member of the Bcl-2/Bcl-xL family (1), is localized in the cytosolic fraction of cells as an inactive precursor (2,3). Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved BID translocates to mitochondria and induces cytochrome c release and mitochondrial damage (2-5). Thus, BID relays an apoptotic signal from the cell surface to mitochondria. However, the precise molecular mechanism for the translocation of the cleaved BID, and for the subsequent release of cytochrome c during apoptosis, is still unclear.
- Wang, K. et al. (1996) Genes Dev 10, 2859-69.
- Luo, X. et al. (1998) Cell 94, 481-90.
- Li, H. et al. (1998) Cell 94, 491-501.
- Gross, A. et al. (1999) J Biol Chem 274, 1156-63.
- Yin, X.M. et al. (1999) Nature 400, 886-91.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
