Product Pathways - Apoptosis / Autophagy
BID Antibody (Mouse Specific) #2003
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | M | Endogenous | 22 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
M=Mouse
Species cross-reactivity is determined by Western blot.
Protocols
- 2003:
- Western Blotting
Specificity / Sensitivity
BID Antibody (Mouse Specific) detects endogenous levels of full length mouse BID protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of mouse BID. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from L929 cells, untreated, etoposide-treated (25 µM) or TNF-α-treated (20 ng/ml), using BID Antibody (Mouse Specific).
Background
The BH3 domain-only protein, BID, a death agonist member of the Bcl-2/Bcl-xL family (1), is localized in the cytosolic fraction of cells as an inactive precursor (2,3). Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved BID translocates to mitochondria and induces cytochrome c release and mitochondrial damage (2-5). Thus, BID relays an apoptotic signal from the cell surface to mitochondria. However, the precise molecular mechanism for the translocation of the cleaved BID, and for the subsequent release of cytochrome c during apoptosis, is still unclear.
- Wang, K. et al. (1996) Genes Dev 10, 2859-69.
- Luo, X. et al. (1998) Cell 94, 481-90.
- Li, H. et al. (1998) Cell 94, 491-501.
- Gross, A. et al. (1999) J Biol Chem 274, 1156-63.
- Yin, X.M. et al. (1999) Nature 400, 886-91.
Application References
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Companion Products
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- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
