Cell Signaling Technology

Product Pathways - Apoptosis

BID Antibody (Mouse Specific) #2003

Applications Reactivity Sensitivity MW (kDa) Source
W M Endogenous 22 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BID Antibody (Mouse Specific) detects endogenous levels of full length mouse BID protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of mouse BID. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from L929 cells, untreated, etoposide-treated (25 µM) or TNF-α-treated (20 ng/ml), using BID Antibody (Mouse Specific).

Background

Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax (1). Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane (2-4). Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation (5-7). Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation (8,9).

  1. Wang, K. et al. (1996) Genes Dev 10, 2859-69.
  2. Luo, X. et al. (1998) Cell 94, 481-90.
  3. Li, H. et al. (1998) Cell 94, 491-501.
  4. Gross, A. et al. (1999) J Biol Chem 274, 1156-63.
  5. Li, P. et al. (1997) Cell 91, 479-89.
  6. Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
  7. Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
  8. Yin, X.M. et al. (1999) Nature 400, 886-91.
  9. Korsmeyer, S.J. et al. (2000) Cell Death Differ 7, 1166-73.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products