Cell Signaling Technology

Product Pathways - Development

FoxP1 Antibody #2005

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P IF-IC F H M (R) Endogenous 82-90 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FoxP1 Antibody detects endogenous level of total FoxP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp666 of human FoxP1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HBP-ALL cells, HCT-15 cells and mouse thymocytes using FoxP1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using FoxP1 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using FoxP1 Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil epithelium using FoxP1 Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF-7 cells using FoxP1 Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using FoxP1 Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red).


Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response, and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).

FoxP1 plays an important role in cardiac valve and B cell development. Targeted deletion of FoxP1 in mice results in embryonic death at E14.5 with severe defects in cardiac outflow development, endocardial cushion morphogenesis, and myocyte proliferation and maturation (6). The FoxP1 gene is located on chromosome 3p14.1 and loss of heterozygosity of this region in multiple cancer types suggests a role for Foxp1 as a tumor suppressor (7). On the other hand, FoxP1 is highly expressed in a variety of B cell malignancies and is frequently involved in chromosome translocation, suggesting that FoxP1 may also act as an oncogene (8,9).

  1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
  2. Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
  3. Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
  4. Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
  5. Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
  6. Wang, B. et al. (2004) Development 131, 4477-87.
  7. Banham, A.H. et al. (2001) Cancer Res 61, 8820-9.
  8. Wlodarska, I. et al. (2005) Leukemia 19, 1299-305.
  9. Goatly, A. et al. (2008) Mod Pathol 21, 902-11.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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