Cell Signaling Technology

Product Pathways - Apoptosis

BID (7A3) Mouse mAb (Human Specific) #2006

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 22 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BID (7A3) Mouse mAb (Human Specific) detects endogenous levels of full length human BID.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of human BID. Antibody is supplied in HEPES buffer with 50% glycerol and less than 0.02% sodium azide.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, A431 and HeLa cells, using BID (7A3) Mouse mAb (Human Specific).

Background

Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax (1). Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane (2-4). Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation (5-7). Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation (8,9).

  1. Wang, K. et al. (1996) Genes Dev 10, 2859-69.
  2. Luo, X. et al. (1998) Cell 94, 481-90.
  3. Li, H. et al. (1998) Cell 94, 491-501.
  4. Gross, A. et al. (1999) J Biol Chem 274, 1156-63.
  5. Li, P. et al. (1997) Cell 91, 479-89.
  6. Zou, H. et al. (1999) J Biol Chem 274, 11549-56.
  7. Saleh, A. et al. (1999) J Biol Chem 274, 17941-5.
  8. Yin, X.M. et al. (1999) Nature 400, 886-91.
  9. Korsmeyer, S.J. et al. (2000) Cell Death Differ 7, 1166-73.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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