Product Pathways - Apoptosis / Autophagy
BID (7A3) Mouse mAb (Human Specific) #2006
PhosphoSitePlus® protein, site, and accession data: Bid
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Endogenous | 22 | Mouse IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2006:
- Western Blotting
Specificity / Sensitivity
BID (7A3) Mouse mAb (Human Specific) detects endogenous levels of full length human BID.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of human BID. Antibody is supplied in HEPES buffer with 50% glycerol and less than 0.02% sodium azide.
Western Blotting
Western blot analysis of extracts from Jurkat, A431 and HeLa cells, using BID (7A3) Mouse mAb (Human Specific).
Background
The BH3 domain-only protein, BID, a death agonist member of the Bcl-2/Bcl-xL family (1), is localized in the cytosolic fraction of cells as an inactive precursor (2,3). Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved BID translocates to mitochondria and induces cytochrome c release and mitochondrial damage (2-5). Thus, BID relays an apoptotic signal from the cell surface to mitochondria. However, the precise molecular mechanism for the translocation of the cleaved BID, and for the subsequent release of cytochrome c during apoptosis, is still unclear.
- Wang, K. et al. (1996) Genes Dev 10, 2859-69.
- Luo, X. et al. (1998) Cell 94, 481-90.
- Li, H. et al. (1998) Cell 94, 491-501.
- Gross, A. et al. (1999) J Biol Chem 274, 1156-63.
- Yin, X.M. et al. (1999) Nature 400, 886-91.
Application References
- Weisberg, E. et al. (2011) PLoS One 6, e25351. Applications: Western Blotting
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
