Product Pathways - Translational Control
eIF4A (C32B4) Rabbit mAb #2013
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W | H M R Mk | Endogenous | 48 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
eIF4A (C32B4) Rabbit mAb detects endogenous levels of total eIF4A protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from residues surrounding Met316 of human eIF4A protein.
Western Blotting
Western blot analysis of extracts from various cell types using eIF4A (C32B4) Rabbit mAb.
Background
A variety of factors contribute to the important biological event of initiation of translation. The eIF4F complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signalling in a translation-independent manner in Drosophila (4,5).
- Rogers, G.W. et al. (2001) J. Biol. Chem. 276, 12598-12608.
- Rogers, G.W. et al. (1999) J. Biol. Chem. 274, 12236-12244.
- Svitkin, Y.V. et al. (2001) RNA 7, 382-394.
- Li, J. and Li, W.X. (2006) Nat. Cell Biol. 8, 1407-1414.
- Affolter, M. and Pyrowolakis, G. (2006) Nat. Cell Biol. 8, 1319-1321.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
