Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - DNA Damage

MSH2 (D24B5) XP® Rabbit mAb #2017

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC F H M Endogenous 100 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MSH2 (D24B5) XP® Rabbit mAb detects endogenous levels of total MSH2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human MSH2.

Western Blotting

Western Blotting

Western blot analysis of extracts of HeLa and NIH/3T3 cells using MSH2 (D24B5) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MSH2 (D24B5) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using MSH2 (D24B5) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using MSH2 (D24B5) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between non-identical DNA sequences and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins and the Rad50-Mre11-NBS1 complex (2).Mutations in MSH2 have been found in a large proportion of hereditary non-polyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations have also been associated with other sporadic tumors.

  1. O'Brien, V. and Brown, R. (2006) Carcinogenesis 27, 682-92.
  2. Wang, Y. et al. (2000) Genes Dev 14, 927-39.
  3. Plotz, G. et al. (2006) J Mol Histol 37, 271-83.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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