Cell Signaling Technology

Product Pathways - Metabolism

Hexokinase I (C35C4) Rabbit mAb #2024

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC H M Endogenous 102 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Hexokinase I (C35C4) Rabbit mAb detects endogenous levels of total hexokinase I protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase I.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Hexokinase I (C35C4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Hexokinase I (C35C4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Hexokinase I (C35C4) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Hexokinase I (C35C4) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

  1. Warburg, O. (1956) Science 123, 309-314.
  2. Semenza, G.L. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 71-103.
  3. Smith, T.A. (2000) Br. J. Biomed. Sci. 57, 170-178.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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