Cell Signaling Technology

Product Pathways - Translational Control

Miwi (D478) Antibody #2025

Applications Reactivity Sensitivity MW (kDa) Source
W M Endogenous 90 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Miwi (D478) Antibody detects endogenous levels of total Miwi protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp478 of mouse Miwi. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various mouse tissues using Miwi (D478) Antibody (upper panel) or β-Actin Antibody #4967 (lower panel).

Background

The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and Homo sapiens (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).

  1. Cox, D.N. et al. (1998) Genes Dev 12, 3715-27.
  2. Kuramochi-Miyagawa, S. et al. (2001) Mech Dev 108, 121-33.
  3. Aravin, A. et al. (2006) Nature 442, 203-7.
  4. Grivna, S.T. et al. (2006) Proc Natl Acad Sci USA 103, 13415-20.
  5. Grivna, S.T. et al. (2006) Genes Dev 20, 1709-14.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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