Product Pathways - Apoptosis
Phospho-Lamin A/C (Ser22) Antibody #2026
|W IF-IC||H M R||Endogenous||69, 78||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-Lamin A/C (Ser22) Antibody detects endogenous levels of lamin A/C only when phosphorylated at Ser22.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser22 of human lamin A/C protein.
Western blot analysis of extracts from HeLa and NIH/3T3 cells, hydroxyurea-treated (4 mM, 20 hours) to induce G1/S phase or paclitaxel-treated (100 nM, 20 hours) or nocodazole-treated (100 ng/ml, 20 hours) to induce G2/M phase, using Phospho-Lamin A/C (Ser22) Antibody (upper) or Lamin A/C Antibody #2032 (lower) as a loading control.
Western blot analysis of extract from G2/M phase HeLa cells untreated or treated with phosphatase, using Phospho-Lamin A/C (Ser22) Antibody (upper) or Lamin A/C Antibody (lower) as loading control.
Confocal immunofluorescent analysis of HeLa cells using Phospho-Lamin A/C (Ser22) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).
Phosphorylation of Lamin A/C at Ser22 was identified in vivo in several cell lines by mass spectrometry analysis in proteomic screens. The surrounding sequence is a typical MAPK/CDK phosphorylation motif, which implicates a role in the cell cycle and mitosis (7-11).
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For Research Use Only. Not For Use In Diagnostic Procedures.