Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

MCP-1 Antibody #2027

Applications Reactivity Sensitivity MW (kDa) Source
W H (Mk) Endogenous 13-15 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MCP-1 Antibody detects endogenous levels of total human MCP-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys35 of human MCP-1. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with human MCP-1, using MCP-1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from the media of A549 cells, untreated or treated with TNF-α #2169 (20 ng/ml, 6 hrs.), using MCP-1 Antibody.

Background

Monocyte chemotactic protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or β) chemokines (1-4). The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis (5), rheumatoid arthritis (6) and atherosclerosis (7). MCP-1 may also contribute to tumor progression and angiogenesis (8). Signaling by MCP-1 is mediated by the G-protein coupled receptor CCR2 (9).

  1. Matsushima, K. et al. (1989) J Exp Med 169, 1485-90.
  2. Furutani, Y. et al. (1989) Biochem Biophys Res Commun 159, 249-55.
  3. Robinson, E.A. et al. (1989) Proc Natl Acad Sci USA 86, 1850-4.
  4. Rollins, B.J. et al. (1988) Proc Natl Acad Sci USA 85, 3738-42.
  5. Gillitzer, R. et al. (1993) J Invest Dermatol 101, 127-31.
  6. Koch, A.E. et al. (1992) J Clin Invest 90, 772-9.
  7. Ylä-Herttuala, S. et al. (1991) Proc Natl Acad Sci USA 88, 5252-6.
  8. Salcedo, R. et al. (2000) Blood 96, 34-40.
  9. Charo, I.F. et al. (1994) Proc Natl Acad Sci USA 91, 2752-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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