Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

MCP-1 Antibody (Mouse Specific) #2029

Applications Reactivity Sensitivity MW (kDa) Source
W M Endogenous 11-13, 20-30 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MCP-1 Antibody (Mouse Specific) detects endogenous levels of total mouse MCP-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys75 of mouse MCP-1. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of recombinant mouse MCP-1 protein using MCP-1 Antibody (Mouse Specific).

Western Blotting

Western Blotting

Western blot analysis of extracts from the media of RAW 264.7 cells, untreated or treated with LPS (1 μg/ml, overnight), using MCP-1 Antibody (Mouse Specific).

Background

Monocyte chemotactic protein-1 (MCP-1), also known as CCL2, monocyte chemotactic activating factor (MCAF) or glioma-derived chemotactic factor-2 (GDCF-2), is the product of the human JE gene and a member of the family of C-C (or β) chemokines (1-4). The predicted molecular weight of MCP-1 protein is 11-13 kDa, but it may migrate at 20-30 kDa due to glycosylation. MCP-1 is secreted by a variety of cell types in response to pro-inflammatory stimuli and was originally described for its chemotactic activity on monocytes. This activity has led to studies demonstrating its role in diseases characterized by monocyte infiltrates such as psoriasis (5), rheumatoid arthritis (6) and atherosclerosis (7). MCP-1 may also contribute to tumor progression and angiogenesis (8). Signaling by MCP-1 is mediated by the G-protein coupled receptor CCR2 (9).

  1. Matsushima, K. et al. (1989) J Exp Med 169, 1485-90.
  2. Furutani, Y. et al. (1989) Biochem Biophys Res Commun 159, 249-55.
  3. Robinson, E.A. et al. (1989) Proc Natl Acad Sci USA 86, 1850-4.
  4. Rollins, B.J. et al. (1988) Proc Natl Acad Sci USA 85, 3738-42.
  5. Gillitzer, R. et al. (1993) J Invest Dermatol 101, 127-31.
  6. Koch, A.E. et al. (1992) J Clin Invest 90, 772-9.
  7. Ylä-Herttuala, S. et al. (1991) Proc Natl Acad Sci USA 88, 5252-6.
  8. Salcedo, R. et al. (2000) Blood 96, 34-40.
  9. Charo, I.F. et al. (1994) Proc Natl Acad Sci USA 91, 2752-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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