Product Pathways - Apoptosis
Lamin A/C Antibody #2032
PhosphoSitePlus® protein, site, and accession data: lamin A/C
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P | H M R (B) | Endogenous | 28, 70 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Lamin A/C Antibody detects endogenous levels of total full length lamin A (and lamin C) (70 kDa), as well as the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230 (28 kDa).
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp230 of human lamin A. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or staurosporine-treated (1 µM), using Lamin A/C Antibody.
Western Blotting
Western blot analysis of extracts from HeLa, Raw 264.7 and PC12 cells, using Lamin A/C Antibody.
IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded human breast tumor, showing staining of the nuclear envelope, using Lamin A/C Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Lamin A/C Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
Background
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).
- Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
- Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
- Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
- Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
- Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
- Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
Application References
- Kim, K.B. et al. (2002) Mol Cancer Ther 1, 177-84. Applications: Western Blotting
- Irwin, M. S. et al. (2003) Chemosensitivity. Cancer Cell. 3, 403-410. Applications: Western Blotting
- Dentin, R. et al. (2004) Hepatic Glucokinase Is Required for the Synergistic Action of ChREBP and SREBP-1c on Glycolytic and Lipogenic Gene Expression. J. Biol. Chem. 279, 20314-20326. Applications: Western Blotting
- Sun, S. et al. (2002) The synthetic retinoid CD437 selectively induces apoptosis in human lung cancer cells while sparing normal human lung epithelial cells. Cancer Res. 62 (8), 2430-2436. Applications: Western Blotting
- Mintzer, R. et al. (2012) PLoS One 7, e30376. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2031 Cleaved Lamin A (Asp230) Antibody
- 2035 Cleaved Lamin A (Small Subunit) Antibody
- 2036 Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
