Product Pathways - Apoptosis / Autophagy

Lamin A/C Antibody #2032

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Applications	Reactivity	MW (kDa)	Source
W IP IHC-P F	H M R (B)	28, 70	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Lamin A/C Antibody detects endogenous levels of total full length lamin A (and lamin C) (70 kDa), as well as the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230 (28 kDa).

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Asp230 of human lamin A. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (40-45 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

Application References

• Kim, K. et al. (2002) Identification of a novel synthetic triterpenoid, that potently induces caspase-mediated apoptosis in human lung cancer cells . Mol. Cancer Ther. 1, 177-184. This article references the use of Lamin A/C Antibody in the following applications: Western Blotting
• Irwin, M. S. et al. (2003) Chemosensitivity. Cancer Cell. 3, 403-410. This article references the use of Lamin A/C Antibody in the following applications: Western Blotting
• Dentin, R. et al. (2004) Hepatic Glucokinase Is Required for the Synergistic Action of ChREBP and SREBP-1c on Glycolytic and Lipogenic Gene Expression. J. Biol. Chem. 279, 20314-20326. This article references the use of Lamin A/C Antibody in the following applications: Western Blotting
• Sun, S. et al. (2002) The synthetic retinoid CD437 selectively induces apoptosis in human lung cancer cells while sparing normal human lung epithelial cells. Cancer Res. 62 (8), 2430-2436. This article references the use of Lamin A/C Antibody in the following applications: Western Blotting

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