Cell Signaling Technology

Product Pathways - Glucose Metabolism

DHCR24/Seladin-1 (C59D8) Rabbit mAb #2033

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P H M Endogenous 54 Rabbit igG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

DHCR24/Seladin-1 (C59D8) Rabbit mAb detects endogenous levels of total DHCR24/Seladin-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) derived from the sequence of human DHCR24/Seladin-1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using DHCR24/Seladin-1 (C59D8) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using DHCR24/Seladin-1 (C59D8F6) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Background

DHCR24/Seladin-1 was identified as a molecular basis for desmosterolosis (1). It encodes for 24-dehydrocholesterol reductase (3β-hydroxysterol ?24-reductase). This enzyme reduces desmosterol in cholesterol biosynthesis (1). Recessive mutations in this gene in desmosterolosis patients lead to a defective enzyme resulting in increased levels of desmosterol (1). DHCR24/Seladin-1 is induced upon oxidative stress and was found to mediate Ras-induced senescence resulting from increased reactive oxygen species (2). Studies further indicate that the level of DHCR24/Seladin-1 is induced in the acute response and reduced in the chronic response to oxidative stress in a cholesterol dependent manner (3). Moreover, overexpression of DHCR24/Seladin-1 bearing two mutations that abolish its reductase acitivity causes the cells to lose protection from oxidative stress (3). These findings thus link the reductase activity of DHCR24/Seladin-1 to its protective role in oxidative stress. This enzyme has also been demonstrated to be a hydrogen peroxide scavenger (4).

  1. Waterham, H.R. et al. (2001) Am J Hum Genet 69, 685-94.
  2. Wu, C. et al. (2004) Nature 432, 640-5.
  3. Kuehnle, K. et al. (2008) Mol Cell Biol 28, 539-50.
  4. Lu, X. et al. (2008) Endocrinology , Epub ahead of print.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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