Product Pathways - Apoptosis / Autophagy
Cleaved Lamin A (Small Subunit) Antibody #2035
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P | H M R | Endogenous | 28 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Cleaved Lamin A (Small Subunit) Antibody detects the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230 by caspase-6. The antibody does not cross-react with full length lamin A or lamin C.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to amino-terminal residues surrounding Asp230 in human lamin A. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3 or C6 cells, untreated or staurosporine-treated (1 µM), using Cleaved Lamin A (Small Subunit) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis using Cleaved Lamin A (Small Subunit) Antibody on SignalSlide(TM) Cleaved Caspase-3 (Asp175) IHC Controls #8104 (Jurkat cell pellets,untreated (left) or etopside treated (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human tonsil using Cleaved Lamin A (Small Subunit) Antibody.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or staurosporine-treated (right), using Cleaved Lamin A (Small Subunit) Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (40-45 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).
- Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
- Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
- Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
- Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
- Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
- Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2032 Lamin A/C Antibody
- 2031 Cleaved Lamin A (Asp230) Antibody
- 2036 Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb
- 9661 Cleaved Caspase-3 (Asp175) Antibody
- 9762 Caspase-6 Antibody
- 9761 Cleaved Caspase-6 (Asp162) Antibody
- 8104 SignalSlide™ Cleaved Caspase-3 (Asp175) IHC Controls
- 8112 SignalStain® Antibody Diluent
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
