Product Pathways - Apoptosis
Cleaved Lamin A (Small Subunit) Antibody #2035
PhosphoSitePlus® protein, site, and accession data: lamin A/C
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P IF-IC | H M R | Endogenous | 28 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Cleaved Lamin A (Small Subunit) Antibody detects the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230 by caspase-6. The antibody does not cross-react with full length lamin A or lamin C.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp230 in human lamin A. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3 or C6 cells, untreated or staurosporine-treated (1 µM), using Cleaved Lamin A (Small Subunit) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis using Cleaved Lamin A (Small Subunit) Antibody on SignalSlide(TM) Cleaved Caspase-3 (Asp175) IHC Controls #8104 (Jurkat cell pellets,untreated (left) or etopside treated (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human tonsil using Cleaved Lamin A (Small Subunit) Antibody.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or staurosporine-treated (right), using Cleaved Lamin A (Small Subunit) Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).
- Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
- Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
- Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
- Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
- Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
- Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
Application References
- Mintzer, R. et al. (2012) PLoS One 7, e30376. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 2032 Lamin A/C Antibody
- 2031 Cleaved Lamin A (Asp230) Antibody
- 2036 Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb
- 8112 SignalStain® Antibody Diluent
- 8104 SignalSlide® Cleaved Caspase-3 (Asp175) IHC Controls
- 9661 Cleaved Caspase-3 (Asp175) Antibody
- 9762 Caspase-6 Antibody
- 9761 Cleaved Caspase-6 (Asp162) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
