Product Pathways - Apoptosis
Cleaved Lamin A (Small Subunit) Antibody #2035
|2035S||100 µl (10 western blots)||---||In Stock||---|
|2035||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||28||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Cleaved Lamin A (Small Subunit) Antibody detects the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230 by caspase-6. The antibody does not cross-react with full length lamin A or lamin C.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp230 in human lamin A. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HeLa, NIH/3T3 or C6 cells, untreated or staurosporine-treated (1 µM), using Cleaved Lamin A (Small Subunit) Antibody.
Immunohistochemical analysis using Cleaved Lamin A (Small Subunit) Antibody on SignalSlide(TM) Cleaved Caspase-3 (Asp175) IHC Controls #8104 (Jurkat cell pellets,untreated (left) or etopside treated (right).
Immunohistochemical analysis of paraffin-embedded human tonsil using Cleaved Lamin A (Small Subunit) Antibody.
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6).
- Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
- Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
- Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
- Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
- Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
- Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
- Mintzer, R. et al. (2012) PLoS One 7, e30376. Applications: Western Blotting.
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