Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb #2036

Applications Reactivity MW (kDa) Source Isotype
W IF-IC H M R 28 Mouse IgG1

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb detects endogenous levels of the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230. The antibody does not cross-react with full length lamin A or C.

Source / Purification

Monoclonal antibody is produced by immunizing mice with a synthetic peptide corresponding to amino-terminal residues surrounding Asp230 of human lamin A.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, untreated or staurosporine-treated (1 µM), and Jurkat cells, untreated or etoposide-treated (25 µM), using Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb.

IF-IC

IF-IC

Immunofluorescent analysis of HeLa cells, untreated (right) or staurosporine-treated (left), using Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb.

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (40-45 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
  6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

Application References

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