Product Pathways - Apoptosis
Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb #2036
|W IF-IC||H M R||Endogenous||28||Mouse IgG1|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb detects endogenous levels of the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230. The antibody does not cross-react with full length lamin A or C.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp230 of human lamin A.
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, untreated or staurosporine-treated (1 µM), and Jurkat cells, untreated or etoposide-treated (25 µM), using Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb.
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).
- Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
- Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
- Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
- Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
- Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
- Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
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For Research Use Only. Not For Use In Diagnostic Procedures.