Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Companion Products

GFP (D5.1) XP® Rabbit mAb (HRP Conjugate) #2037

Applications Reactivity Sensitivity MW (kDa) Isotype
W All Transfected Only 27 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

Specificity / Sensitivity

GFP (D5.1) XP® Rabbit mAb (HRP Conjugate) detects GFP-tagged proteins exogenously expressed in cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP. This antibody was conjugated to HRP under optimal conditions.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCC827 cells, mock transfected or transfected with GFP, using GFP (D5.1) XP® Rabbit mAb (HRP Conjugate).

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1).

  1. Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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