Cell Signaling Technology

Product Pathways - Companion Products

Myc-Tag (9B11) Mouse mAb (HRP Conjugate) #2040

Applications Reactivity Sensitivity MW (kDa) Isotype
W Transfected Only 65 Mouse IgG2a

Applications Key:  W=Western Blotting
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Myc-Tag (9B11) Mouse mAb (HRP Conjugate) detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). This antibody was conjugated to HRP under optimal conditions.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with Myc-chorionic somatomammotropin hormone-like 1 (CSL), using Myc-Tag (9B11) Mouse mAb (HRP Conjugate).

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).

  1. Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products