Product Pathways - Apoptosis / Autophagy
XIAP Antibody #2042
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M Mk | Endogenous | 53 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
XIAP Antibody detects endogenous levels of total XIAP protein. The antibody does not cross-react with other inhibitors of apoptosis.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to residues surrounding amino acid 240 of human XIAP. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa (human), C6 (rat) and BaF3 (mouse) cell lines, using XIAP Antibody.
Background
The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of the family include c-IAP1, c-IAP2, XIAP, Surivivin, Livin and NAIP. Overexpression of IAP family members, particularly Survivin and Livin, in cancer cell lines and primary tumors suggest an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7 and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks its interaction with caspase-9, thereby allowing the processing and activation of the caspase (7).
- Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
- Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
- Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
- Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
- Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
- Deveraux, Q.L. et al. (1997) Nature 388, 300-4.
- Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
Application References
- Guegan, C. et al. (2001) Recruitment of the mitochondrial-dependent apoptotic pathway in amyotrophic lateral sclerosis. J. Neurosci. 2 (17), 6569-6576. This article references the use of XIAP Antibody in the following applications: Western Blotting
- Rosato, R. R. et al. (2003) Simultaneous activation of the intrinsic and extrinsic pathways by histone deacetylase (HDAC) inhibitors and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) synergistically induces mitochondrial damage and apoptosis in human leukemia cells. Mol. Cancer Ther. 2, 1273-1284. This article references the use of XIAP Antibody in the following applications: Western Blotting
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Companion Products
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- 9662 Caspase-3 Antibody
- 9746 Caspase-8 (1C12) Mouse mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 6446 SignalSilence® XIAP siRNA (Human specific)
- 6445 SignalSilence® XIAP siRNA Kit (Human Specific)
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
