Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

XIAP Antibody #2042

Applications Reactivity Sensitivity MW (kDa) Source
W H M Mk Endogenous 53 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

XIAP Antibody detects endogenous levels of total XIAP protein. The antibody does not cross-react with other inhibitors of apoptosis.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to residues surrounding amino acid 240 of human XIAP. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa (human), C6 (rat) and BaF3 (mouse) cell lines, using XIAP Antibody.

Background

The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of the family include c-IAP1, c-IAP2, XIAP, Surivivin, Livin and NAIP. Overexpression of IAP family members, particularly Survivin and Livin, in cancer cell lines and primary tumors suggest an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7 and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks its interaction with caspase-9, thereby allowing the processing and activation of the caspase (7).

  1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
  2. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
  3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
  4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
  5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
  6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.
  7. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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