Product Pathways - Companion Products
DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (HRP Conjugate) #2044
| Applications | Reactivity | Sensitivity | Source |
|---|---|---|---|
| W | All | Transfected Only | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 2044:
- Western Blotting
Specificity / Sensitivity
DYKDDDDK Tag Antibody (HRP Conjugate) detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino or carboxy terminus of targeted proteins. The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography. The antibody was conjugated to HRP under optimal conditions.
Western Blotting
Western blot analysis of extracts from COS cells, untransfected (-) or transfected with DYKDDDDK-tagged Fox03 (+), using DYKDDDDK Tag Antibody (HRP Conjugate).
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2272 Myc-Tag Antibody
- 2276 Myc-Tag (9B11) Mouse mAb
- 2365 His-Tag Antibody
- 2367 HA-Tag (6E2) Mouse mAb
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2040 Myc-Tag (9B11) Mouse mAb (HRP Conjugate)
- 2999 HA-Tag (6E2) Mouse mAb (HRP Conjugate)
- 2037 GFP (D5.1) XP® Rabbit mAb (HRP Conjugate)
- 7075 Anti-biotin, HRP-linked Antibody
Anti-FLAG® is a registered trademark of Sigma-Aldrich.
For Research Use Only. Not For Use In Diagnostic Procedures.
