Product Pathways - Companion Products
DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) (HRP Conjugate) #2044
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Specificity / Sensitivity
DYKDDDDK Tag Antibody (HRP Conjugate) detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino or carboxy terminus of targeted proteins. The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography. The antibody was conjugated to HRP under optimal conditions.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).
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Anti-FLAG® is a registered trademark of Sigma-Aldrich.
For Research Use Only. Not For Use In Diagnostic Procedures.