Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

XIAP (3B6) Rabbit mAb #2045

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Mk Endogenous 53 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

XIAP (3B6) Rabbit mAb detects endogenous levels of XIAP. No cross reactivity was observed with other IAP family members.

Source / Purification

Rabbit monoclonal antibodies are prepared from spleens obtained from rabbits immunized with a synthetic peptide (KLH-coupled) corresponding to a region surrounding serine 245 of human XIAP.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, Raji and COS cell lines, using XIAP (3B6) Rabbit mAb.

Background

The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of the family include c-IAP1, c-IAP2, XIAP, surivivin, livin and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggest an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7 and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks its interaction with caspase-9, thereby allowing the processing and activation of the caspase (7).

  1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
  2. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
  3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
  4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
  5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
  6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.
  7. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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