Cell Signaling Technology

Product Pathways - Neuroscience

NGF Antibody #2046

Applications Reactivity Sensitivity MW (kDa) Source
W H M Recombinant 13 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NGF Antibody detects recombinant NGF protein. It does not cross-react with other proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr146 of human NGF.

Western Blotting

Western Blotting

Western blot analysis of various concentrations of recombinant human NGF protein using NGF Antibody.

Background

Nerve growth factor (NGF) is a small, secreted protein and member of the neurotrophin family of growth factors that promote neuronal cell survival and differentiation (1). Producing cells release NGF that bind and activate TrkA high affinity receptors to mediate NGF-driven signaling. NGF also binds to a low affinity p75 (NTR) receptors, which belong to the death receptor family (2). Although NGF has been classically described as favoring neuron survival and differentiation, nerve growth factor can promote apoptosis in cells that contain p75 (NTR) and lack TrkA. NGF can induce neuron death in a variety of neurodegenerative conditions, including Alzheimer disease (3). Besides its neurotrophic actions, NGF has an effect on non-neuronal cells and may help mediate inflammation, angiogenesis, and stimulate breast cancer cell growth (4-6). NGF signaling is looking increasingly promising as potential drug targets for diseases.

  1. Lessmann, V. et al. (2003) Prog Neurobiol 69, 341-74.
  2. Neet, K.E. and Campenot, R.B. (2001) Cell Mol Life Sci 58, 1021-35.
  3. Lorigados-Pedre, L. and Bergado-Rosado, J. (2004) Rev Neurol 38, 957-71.
  4. Frossard, N. et al. (2004) Eur J Pharmacol 500, 453-65.
  5. Nico, B. et al. (2008) Microvasc Res 75, 135-41.
  6. Descamps, S. et al. (2001) J Biol Chem 276, 17864-70.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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