Cell Signaling Technology

Product Pathways - Metabolism

Tug Antibody #2049

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 70-75 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Tug Antibody detects endogenous levels of total Tug protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids around residue Val542 of human Tug. Antibodies are purified by Protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Tug Antibody.

Background

Tug (Tether containing UBX domain for GLUT4), also known as ASPL, ASPSCR1, RCC17, UBXD9, UBXN9, was first identified as a chromosomal translocation partner for TFE3 in patients with Alveolar soft part sarcoma (1) and contains an UBX-like domain in its C-terminal region. Tug is found to tether GLUT4 in intracellular vesicles and to release GLUT4 for cell surface translocation upon insulin stimulation (2). Stable Tug depletion or expression of a dominant negative form stimulates GLUT4 redistribution (3).

  1. Ladanyi, M. et al. (2001) Oncogene 20, 48-57.
  2. Bogan, J.S. et al. (2003) Nature 425, 727-33.
  3. Yu, C. et al. (2007) J Biol Chem 282, 7710-22.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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