Product Pathways - Metabolism
Tug Antibody #2049
|W IP||H M R Mk||Endogenous||70-75||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Tug Antibody detects endogenous levels of total Tug protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids around residue Val542 of human Tug. Antibodies are purified by Protein A and peptide affinity chromatography.
Tug (Tether containing UBX domain for GLUT4), also known as ASPL, ASPSCR1, RCC17, UBXD9, UBXN9, was first identified as a chromosomal translocation partner for TFE3 in patients with Alveolar soft part sarcoma (1) and contains an UBX-like domain in its C-terminal region. Tug is found to tether GLUT4 in intracellular vesicles and to release GLUT4 for cell surface translocation upon insulin stimulation (2). Stable Tug depletion or expression of a dominant negative form stimulates GLUT4 redistribution (3).
- Ladanyi, M. et al. (2001) Oncogene 20, 48-57.
- Bogan, J.S. et al. (2003) Nature 425, 727-33.
- Yu, C. et al. (2007) J Biol Chem 282, 7710-22.
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For Research Use Only. Not For Use In Diagnostic Procedures.