Product Pathways - Ca / cAMP / Lipid Signaling
Phospho-PKD/PKCμ (Ser744/748) Antibody #2054
|W||H M R Mk||Endogenous||115||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-PKD/PKCμ (Ser744/748) Antibody detects PKD1/PKCμ only when dually phosphorylated at serines 744 and 748. This antibody may also cross-react with isoforms PKD2 and PKD3/PKCη in some species.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser744/748 of mouse PKD. Antibodies are purified by protein A and peptide affinity chromatography.
Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6).
- Nishizuka, Y. (1984) Nature 308, 693-698.
- Keranen, L.M. (1995) Curr. Biol. 5, 1394-1403.
- Valverde, A.M. et al. (1994) Proc. Natl. Acad. Sci. 91, 8572-8576.
- Johannes, F.J. et al. (1994) J. Biol. Chem. 269, 6140-6148.
- Iglesias, T. et al. (1998) J. Biol. Chem. 273, 27662-27667.
- Matthews, S.A. et al. (1999) J. Biol. Chem. 274, 26543-26549.
- Wood, C.D. et al. (2005) J Biol Chem 280, 6245-51.
- Amadesi, S. et al. (2009) J Comp Neurol 516, 141-56.
- Zhukova, E. et al. (2001) Activation loop Ser744 and Ser748 in protein kinase D are transphosphorylated in vivo. J Biol Chem 276 (43), 40298-40305. Applications: Western Blotting
- Tan, M. et al. (2003) Thrombin rapidly induces protein kinase D phosphorylation, and protein kinase C delta mediates the activation. J. Biol. Chem. 278, 2824-2828. Applications: Western Blotting
- Waldron, R. T. et al. (2001) Protein kinase D potentiates DNA synthesis and cell proliferation induced by bombesin, vasopressin, or phorbol esters in Swiss 3T3 cells. J Biol Chem 276 (35), 32606-32615. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.