Cell Signaling Technology

Product Pathways - Metabolism

ENPP1 (L520) Antibody #2061

Applications Reactivity Sensitivity MW (kDa) Source
W IP H R (M) Endogenous 140 kDa (Human), 110 kDa (Rodent) Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ENPP1 (L520) Antibody detects endogenous levels of total ENPP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu520 of human ENPP1. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from rat KNRK and human HepG2 cells using ENPP1 (L520) Antibody.

Background

Ectonucleotide pyrophosphatase-phosphodiesterase 1 (ENPP1) is a single-pass, type II transmembrane protein primarily involved in ATP hydrolysis at the plasma membrane. Targeting of ENPP1 to the basolateral cell surface relies on the presence of a carboxy-terminal di-leucine-based signal (1). ENPP1 plays important roles in bone mineralization and soft tissue calcification (2-5). Mutations in the corresponding ENPP1 gene cause generalized arterial calcification in infancy (GACI) and idiopathic infantile arterial calcification (IIAC) (6,7). ENPP1 inhibits insulin receptor function and overexpression of this enzyme causes insulin resistance and glucose intolerance in mice (8,9). Genetic variants of ENPP1 have been associated with obesity and type 2 diabetes (10-12).

  1. Bello, V. et al. (2001) Mol Biol Cell 12, 3004-15.
  2. Okawa, A. et al. (1998) Nat Genet 19, 271-3.
  3. Nakamura, I. et al. (1999) Hum Genet 104, 492-7.
  4. Harmey, D. et al. (2004) Am J Pathol 164, 1199-209.
  5. Hessle, L. et al. (2002) Proc Natl Acad Sci USA 99, 9445-9.
  6. Rutsch, F. et al. (2003) Nat Genet 34, 379-81.
  7. Ruf, N. et al. (2005) Hum Mutat 25, 98.
  8. Dong, H. et al. (2005) Diabetes 54, 367-72.
  9. Maddux, B.A. et al. (2006) Am J Physiol Endocrinol Metab 290, E746-9.
  10. Meyre, D. et al. (2005) Nat Genet 37, 863-7.
  11. Keene, K.L. et al. (2008) Diabetes 57, 1057-62.
  12. Bacci, S. et al. (2007) Curr Opin Clin Nutr Metab Care 10, 403-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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