Cell Signaling Technology

Product Pathways - Development

WIF1 Antibody #2064

Applications Reactivity Sensitivity MW (kDa) Source
W M (H) Transfected Only 42 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

WIF1 Antibody detects transfected levels of total WIF1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln166 of human WIF1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, mock transfected or transfected with a mouse WIF1 construct, using WIF1 Antibody.

Background

WIF1 (Wnt inhibitory factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity (1). It contains an N-terminal WIF domain and five EGF-like repeats (2). The WIF1 ortholog in Drosophila, Shifted, is required for Hedgehog stability and diffusion (3,4). It has been reported that WIF1 expression is downregulated in many types of cancers (5-8).

  1. Hsieh, J.C. et al. (1999) Nature 398, 431-6.
  2. Liepinsh, E. et al. (2006) J Mol Biol 357, 942-50.
  3. Gorfinkiel, N. et al. (2005) Dev Cell 8, 241-53.
  4. Glise, B. et al. (2005) Dev Cell 8, 255-66.
  5. Wissmann, C. et al. (2003) J Pathol 201, 204-12.
  6. Taniguchi, H. et al. (2005) Oncogene 24, 7946-52.
  7. Mazieres, J. et al. (2004) Cancer Res 64, 4717-20.
  8. Mazieres, J. et al. (2005) Cancer Lett 222, 1-10.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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