Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Translational Control

MRPL11 (D68F2) XP® Rabbit mAb #2066

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC H M R Mk Endogenous 21 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MRPL11 (D68F2) XP® Rabbit mAb detects endogenous levels of total MRPL11 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human MRPL11.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using MRPL11 (D68F2) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon using MRPL11 (D68F2) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MRPL11 (D68F2) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using MRPL11 (D68F2) XP® Rabbit mAb (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

A subset of mitochondrial proteins are synthesized on the ribosomes within mitochondria (1). The 55S mammalian mitochondrial ribosomes are composed of a 28S small subunit and a 39S large subunit (1). Over 40 protein components have been identified from the large subunit of the human mitochondrial ribosome (1). The mitochondrial ribosomal protein L11 (MRPL11) is one such component (1). In animals, plants and fungi, this protein is translated from a gene in the nuclear genome (2).

  1. Koc, E.C. et al. (2001) J Biol Chem 276, 43958-69.
  2. Handa, H. et al. (2001) Mol Genet Genomics 265, 569-75.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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