Product Pathways - Translational Control
MRPL11 (D68F2) XP® Rabbit mAb #2066
PhosphoSitePlus® protein, site, and accession data: MRPL11
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H M R Mk | Endogenous | 21 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
MRPL11 (D68F2) XP® Rabbit mAb detects endogenous levels of total MRPL11 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human MRPL11.
Western Blotting
Western blot analysis of extracts from various cell types using MRPL11 (D68F2) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon using MRPL11 (D68F2) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MRPL11 (D68F2) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using MRPL11 (D68F2) XP® Rabbit mAb (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
A subset of mitochondrial proteins are synthesized on the ribosomes within mitochondria (1). The 55S mammalian mitochondrial ribosomes are composed of a 28S small subunit and a 39S large subunit (1). Over 40 protein components have been identified from the large subunit of the human mitochondrial ribosome (1). The mitochondrial ribosomal protein L11 (MRPL11) is one such component (1). In animals, plants and fungi, this protein is translated from a gene in the nuclear genome (2).
- Koc, E.C. et al. (2001) J Biol Chem 276, 43958-69.
- Handa, H. et al. (2001) Mol Genet Genomics 265, 569-75.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
