Cell Signaling Technology

Product Pathways - Translational Control

eIF4E (C46H6) Rabbit mAb #2067

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P H M R Mk Endogenous 25 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eIF4E (C46H6) Rabbit mAb detects endogenous levels of total eIF4E protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human eIF4E protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using eIF4E (C46H6) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® eIF4E siRNA I #6311 or SignalSilence® eIF4E siRNA II #6554 (+), using eIF4E (C46H6) Rabbit mAb #2067 and α-Tubulin (11H10) Rabbit mAb #2125. The eIF4E (C46H6) Rabbit mAb confirms silencing of eIF4E expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of eIF4E siRNA.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using eIF4E (C46H6) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using eIF4E (C46H6) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Background

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.

  1. Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847.
  2. Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
  3. Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880.
  4. Pyronnet, S. et al. (1999) EMBO J. 18, 270-279.
  5. Raught, B. et al. (2000) EMBO J. 19, 434-444.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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