Product Pathways - Translational Control
eIF4E (C46H6) Rabbit mAb #2067
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P | H M R Mk | Endogenous | 25 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
eIF4E (C46H6) Rabbit mAb detects endogenous levels of total eIF4E protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) derived from human eIF4E protein.
Western Blotting
Western blot analysis of extracts from various cell lines using eIF4E (C46H6) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using eIF4E (C46H6) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using eIF4E (C46H6) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Background
Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148 and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.
- Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847.
- Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
- Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880.
- Pyronnet, S. et al. (1999) EMBO J. 18, 270-279.
- Raught, B. et al. (2000) EMBO J. 19, 434-444.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
