Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Axin1 (C95H11) Rabbit mAb #2074

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P H Mk Endogenous 110 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Axin1 (C95H11) Rabbit mAb detects the endogenous levels of total Axin1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein of human Axin1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Axin1 (C95H11) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Axin1 (C95H11) Rabbit mAb.

Background

Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).

  1. Luo, W. and Lin, S.C. Neurosignals 13, 99-113.
  2. Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236.
  3. Zeng, X. et al. (2005) Nature 438, 873-877.
  4. Davidson, G. et al. (2005) Nature 438, 867-872.
  5. Zhang, Y. et al. (1999) J. Biol. Chem. 274, 35247-35254.
  6. Chia, I.V. and Costantini, F. (2005) Mol. Cell Biol. 25, 4371-4376.
  7. Jho, E.H. et al. (2002) Mol. Cell Biol. 22, 1172-1183.
  8. Lustig, B. et al. (2002) Mol. Cell Biol. 22, 1184-1193.
  9. Leung, J.Y. et al. (2002) J. Biol. Chem. 277, 21657-21665.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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