Cell Signaling Technology

Product Pathways - Neuroscience

Mena Antibody #2075

Applications Reactivity MW (kDa) Source
W H R (M) 80 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Mena Antibody detects endogenous levels of total Mena protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) of human Mena. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from C6 and A431 cells using Mena Antibody.

Background

Mena (mammalian enabled), EVL, and VASP are members of the Ena/VASP family, which is involved in controlling cell shape and cell movement by shielding actin filaments from capping proteins (1). Ena/Vasp proteins have three specific domains: an amino-terminal EVH1 domain controlling protein localization; a central proline-rich domain mediating interactions both with SH3 and WW domain containing proteins including profilin, and a carboxy-terminal domain causing tetramerization and binding to actin (2). Mena interacts with actin filaments at the growing ends localizing to lamellipodia and to tips of growth cone filopodia in neurons. Axons projecting from interhemispheric cortico-cortical neurons are misrouted in newborn, homozygous Mena knock-out mice (3). Mena is phosphorylated at Ser236 by PKA promoting filopodial formation and elongation in the growth cone (4).

  1. Gertler, F.B. et al. (1996) Cell 87, 227-39.
  2. Small, J.V. (2008) Nat Cell Biol 10, 118-20.
  3. Lanier, L.M. et al. (1999) Neuron 22, 313-25.
  4. Lebrand, C. et al. (2004) Neuron 42, 37-49.

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