Cell Signaling Technology

Product Pathways - Neuroscience

TMP21 Antibody #2076

Applications Reactivity Sensitivity MW (kDa) Source
W H M Mk (R) Endogenous 19 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TMP21 Antibody detects endogenous levels of total TMP21 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human TMP21. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from U87-MG, Neuro2A and CAD cells using TMP21 Antibody.

Background

TMP21, a type I transmembrane protein, is a member of the p24 cargo protein family, which is highly enriched in the ER, the Golgi and coat protein (COP) I and II transport vesicles (1,2). TMP21 is involved in protein transport and vesicular targeting. In particular, TMP21 influences APP trafficking by stabilizing nascent APP. The absence of TMP21 leads to enhanced maturation and cell surface accumulation of APP (3). In addition, TMP21 is a non-essential component of the γ-secretase complex with the potential to modulate γ-secretase mediated cleavage and Aβ production without having an effect on ε-secretase activity (4).

  1. Blum, R. et al. (1996) J Biol Chem 271, 17183-9.
  2. Blum, R. et al. (1999) J Cell Sci 112 ( Pt 4), 537-48.
  3. Vetrivel, K.S. et al. (2007) Mol Neurodegener 2, 4.
  4. Chen, F. et al. (2006) Nature 440, 1208-12.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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