Product Pathways - Protein Translation
Miwi (G82) Antibody #2079
|2079S||100 µl (10 western blots)||---||In Stock||---|
|2079||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen)
Specificity / Sensitivity
Miwi (G82) Antibody detects endogenous levels of total Miwi protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Gly82 of mouse Miwi. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from various mouse tissues using Miwi (G82) Antibody (upper panel) or β-Actin Antibody (#4967) (lower panel).
Immunohistochemical analysis of paraffin-embedded mouse testis using Miwi (G82) Antibody.
Confocal immunofluorescent analysis of mouse testis using Miwi (G82) Antibody (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and Homo sapiens (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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