Product Pathways - Translational Control
Miwi (G82) Antibody #2079
PhosphoSitePlus® protein, site, and accession data: PIWIL1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-F | M | Endogenous | 90 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Miwi (G82) Antibody detects endogenous levels of total Miwi protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Gly82 of mouse Miwi. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various mouse tissues using Miwi (G82) Antibody (upper panel) or β-Actin Antibody (#4967) (lower panel).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse testis using Miwi (G82) Antibody.
IF-F
Confocal immunofluorescent analysis of mouse testis using Miwi (G82) Antibody (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and Homo sapiens (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).
- Cox, D.N. et al. (1998) Genes Dev 12, 3715-27.
- Kuramochi-Miyagawa, S. et al. (2001) Mech Dev 108, 121-33.
- Aravin, A. et al. (2006) Nature 442, 203-7.
- Grivna, S.T. et al. (2006) Proc Natl Acad Sci USA 103, 13415-20.
- Grivna, S.T. et al. (2006) Genes Dev 20, 1709-14.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
