Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Tyrosine Kinase / Adaptors

EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb #2085

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-P IF-IC F H Endogenous 175 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-P=Immunofluorescence (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb detects endogenous levels of EGFR E746-A750del mutant protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to E746-A750del mutant sequence of human EGFR.

Western Blotting

Western Blotting

Western blot analysis of extracts from various EGFR expressing cell lines, showing the specificity of detection of mutant EGFR (E746-A750del) protein, using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (upper), and total EGFR control antibody (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded H1975 (left, EGFR L858R positive) and H1650 xenografts (right, EGFR deletion positive) using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin embedded human lung carcinoma of known mutational status using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (upper) and total EGFR (D38B1) XP® Rabbit mAb #4267 (lower). EGFR L858R positive lung (left), EGFR deletion positive lung sections (right).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Kyse450 cells (wildtype, blue) and HCC827 cells (exon 19 deletion (E746-A750del, green) using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HCC827, H1650 and Kyse450 cells using EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-P

IF-P

Confocal immunofluorescent analysis of paraffin-embedded H3255, H1975, HCC827 and H1650 xenografts using EGF Receptor (E746-A750del Specific) (6B6) XP® Rabbit mAb (upper, green) and EGFR (D38B1) XP® Rabbit mAb #4267 (lower, green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarinomas that respond to EGFR inhibitors, such as gefinitib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.

  1. Lynch, T.J. et al. (2004) N Engl J Med 350, 2129-39.
  2. Pao, W. et al. (2004) Proc Natl Acad Sci USA 101, 13306-11.
  3. Haber, D.A. et al. (2005) Cold Spring Harb Symp Quant Biol 70, 419-26.
  4. Kosaka, T. et al. (2004) Cancer Res 64, 8919-23.
  5. Riely, G.J. et al. (2006) Clin Cancer Res 12, 7232-41.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

The manufacture, use, sale and import of this product is within the scope of one or more intellectual property rights (including patents and patent applications) owned or controlled by Cell Signaling Technology. The purchase of this product conveys to the buyer a non-transferrable right to use the purchased product only in research conducted by the buyer. The sale of the product is expressly conditioned on the buyer not using the products or its components (1) to analyze or reverse engineer the product for its chemical/physical properties and composition (including e.g., identification of the sequence); (2) in manufacturing; (3) to provide a service, information, or data to an unaffiliated third party for payment; (4) for therapeutic, diagnostic or prophylactic purposes; (5) resale, whether or not such product are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Cell Signaling Technology, Inc. Business Development at busdev@cellsignal.com.

For Research Use Only. Not For Use In Diagnostic Procedures.

Products