Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Centrin-2 Antibody #2091

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk Endogenous 20 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Centrin-2 Antibody detects endogenous levels of total centrin-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human centrin-2. Antibodies are purified using protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Centrin-2 Antibody.

Background

Centrins are small conserved microtubule nucleating proteins localized to centrosomes, also known as microtubule organizing centers (MTOC), in eukaryotic cells. Centrin-1 is associated with cells that have cilia and flagella, whereas centrin-2 and -3 are ubiquitously expressed and important in centrosome duplication during cell division, as well as the structure and function of the MTOC (1-3). Human centrin-2 has also been shown to localize to nuclear pores and to have a role in regulation of mRNA export (4). The yeast ortholog of centrin-2, CDC31, plays a part in control of protein degradation and sensitivity to DNA damage (5).

  1. Salisbury, J.L. (2007) J Cell Physiol 213, 420-8.
  2. Salisbury, J.L. et al. (2002) Curr Biol 12, 1287-92.
  3. Middendorp, S. et al. (2000) J Cell Biol 148, 405-16.
  4. Resendes, K.K. et al. (2008) Mol Cell Biol 28, 1755-69.
  5. Chen, L. and Madura, K. (2008) Mol Cell Biol 28, 1829-40.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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