Product Pathways - Cytoskeletal Signaling
Centrin-2 Antibody #2091
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H Mk | 20 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Centrin-2 Antibody detects endogenous levels of total centrin-2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the amino terminus of human centrin-2. Antibodies are purified using protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using Centrin-2 Antibody.
Background
Centrins are small conserved microtubule nucleating proteins localized to centrosomes, also known as microtubule organizing centers (MTOC), in eukaryotic cells. Centrin-1 is associated with cells that have cilia and flagella, whereas centrin-2 and -3 are ubiquitously expressed and important in centrosome duplication during cell division, as well as the structure and function of the MTOC (1-3). Human centrin-2 has also been shown to localize to nuclear pores and to have a role in regulation of mRNA export (4). The yeast ortholog of centrin-2, CDC31, plays a part in control of protein degradation and sensitivity to DNA damage (5).
- Salisbury, J.L. (2007) J Cell Physiol 213, 420-8.
- Salisbury, J.L. et al. (2002) Curr Biol 12, 1287-92.
- Middendorp, S. et al. (2000) J Cell Biol 148, 405-16.
- Resendes, K.K. et al. (2008) Mol Cell Biol 28, 1755-69.
- Chen, L. and Madura, K. (2008) Mol Cell Biol 28, 1829-40.
Application References
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