Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

SCF (C19H6) Rabbit mAb #2093

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P F H Endogenous 18 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SCF (C19H6) Rabbit mAb detects endogenous levels of SCF by immunohistochemical staining and by flow cytometry. Western blotting detects recombinant SCF.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids in the N-terminal sequence of human SCF.

Western Blotting

Western Blotting

Western blot analysis of recombinant SCF (5 ng) using SCF (C19H6) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using SCF (C19H6) RmAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of U-87 MG cells using SCF (C19H6) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).


Background

Stem cell factor (SCF) is a growth factor that is essential for hematopoiesis, melanogenesis and fertility. SCF is also known as mast cell growth factor (MCGF), steel factor (SLF), or kit ligand (KL) (1-3). SCF mediates its biological effects by binding to and activating c-Kit (4). SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates and activation of multiple signaling pathways (5,6). SCF/c-Kit may take part in the growth control of human malignancies (7).

  1. Smith, M.A. et al. (2001) Blood Rev. 15, 191-197.
  2. Linnekin, D. (1999) Int. J. Biochem. Cell Biol. 31, 1053-1074.
  3. Huang, E. et al. (1990) Cell 63, 225-233.
  4. Jiang, X. et al. (2000) EMBO J. 19, 3192-3203.
  5. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31, 1037-1051.
  6. Ueda, S. et al. (2002) Blood 99, 3342-3349.
  7. Attoub, S. et al. (2002) Cancer Res. 62, 4879-4883.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products