Product Pathways - Cytoskeletal Signaling
Rab7 Antibody #2094
Have you tried your application using our XP® monoclonal antibodies? Try product: 9367
PhosphoSitePlus® protein, site, and accession data: Rab7
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 23 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2094:
- Western Blotting
Specificity / Sensitivity
Rab7 Antibody detects endogenous levels of total Rab7 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Asp193 of human Rab7. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from SK-MEL-28 and NIH/3T3 cells using Rab7 Antibody.
Background
Rab7 and Rab9 are members of the Ras superfamily of small Rab GTPases (1). Both proteins are located in late endosomes, but exert different functions. Rab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes (2,3). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation (3,4), and maturation of phagosome and autophagic vacuoles (4-6). Rab9 interacts with its effector proteins p40 and TIP47 (7,8) to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network (9,10).
- Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
- Feng, Y. et al. (1995) J Cell Biol 131, 1435-52.
- Méresse, S. et al. (1995) J Cell Sci 108 ( Pt 11), 3349-58.
- Ceresa, B.P. and Bahr, S.J. (2006) J Biol Chem 281, 1099-106.
- Jäger, S. et al. (2004) J Cell Sci 117, 4837-48.
- Méresse, S. et al. (1999) EMBO J 18, 4394-403.
- Díaz, E. et al. (1997) J Cell Biol 138, 283-90.
- Barbero, P. et al. (2002) J Cell Biol 156, 511-8.
- Lombardi, D. et al. (1993) EMBO J 12, 677-82.
- Riederer, M.A. et al. (1994) J Cell Biol 125, 573-82.
Application References
- Tong, Y. et al. (2012) Mol Neurodegener 7, 2. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
