Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Rab9 Antibody #2095

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 23 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Rab9 Antibody detects endogenous levels of total Rab9 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn184 of human Rab9. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Rab9 Antibody.

Background

Rab7 and Rab9 are members of the Ras superfamily of small Rab GTPases (1). Both proteins are located in late endosomes but exert different functions. Rab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes (2,3). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation (3,4), and maturation of phagosome and autophagic vacuoles (4-6). Rab9 interacts with its effector proteins p40 and TIP47 (7,8) to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network (9,10).

  1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
  2. Feng, Y. et al. (1995) J Cell Biol 131, 1435-52.
  3. Méresse, S. et al. (1995) J Cell Sci 108 ( Pt 11), 3349-58.
  4. Ceresa, B.P. and Bahr, S.J. (2006) J Biol Chem 281, 1099-106.
  5. Jäger, S. et al. (2004) J Cell Sci 117, 4837-48.
  6. Méresse, S. et al. (1999) EMBO J 18, 4394-403.
  7. Díaz, E. et al. (1997) J Cell Biol 138, 283-90.
  8. Barbero, P. et al. (2002) J Cell Biol 156, 511-8.
  9. Lombardi, D. et al. (1993) EMBO J 12, 677-82.
  10. Riederer, M.A. et al. (1994) J Cell Biol 125, 573-82.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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