Cell Signaling Technology

Product Pathways - Translational Control

eIF2α (L57A5) Mouse mAb #2103

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H M R Mk Endogenous 38 Mouse IgG2b

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eIF2α (L57A5) Mouse mAb detects endogenous levels of total eIF2α protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with purified recombinant human eIF2α.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using eIF2α (L57A5) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using eIF2α (L57A5) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eIF2α (L57A5) Mouse mAb.


Background

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).

  1. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
  2. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
  3. Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
  4. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
  5. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
  6. Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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