Cell Signaling Technology

Product Pathways - Metabolism

Hexokinase II Antibody #2106

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk Endogenous 102 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Hexokinase II Antibody detects endogenous levels of total hexokinase (isoform II) protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase (isoform II). Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, SH-SY5Y and LN18 cells using Hexokinase II Antibody.

Background

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

  1. Warburg, O. (1956) Science 123, 309-314.
  2. Semenza, G.L. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 71-103.
  3. Smith, T.A. (2000) Br. J. Biomed. Sci. 57, 170-178.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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