Product Pathways - Glucose Metabolism
Hexokinase II Antibody #2106
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H Mk | Endogenous | 102 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Hexokinase II Antibody detects endogenous levels of total hexokinase (isoform II) protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human hexokinase (isoform II). Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa, SH-SY5Y and LN18 cells using Hexokinase II Antibody.
IP
Immunoprecipitation of Hexokinase II from untreated Cos cell extracts using Hexokinase II antibody (Lane 1). Lane 2: No antibody control. Lane 3: Input control.
Background
Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III and IV (glucokinase), have been identified. Hexokinases I, II and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with the rapid cell growth and proliferation (2,3).
- Warburg, O. (1956) Science 123, 309-314.
- Semenza, G.L. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 71-103.
- Smith, T.A. (2000) Br. J. Biomed. Sci. 57, 170-178.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
