Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

Non-phospho-Src (Tyr527) Antibody #2107

Applications Reactivity MW (kDa) Source
W H M R (C) 60 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  C=Chicken
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Non-phospho-Src (Tyr527) Antibody detects endogenous levels of Src only when dephosphorylated at tyrosine 527. It may also cross-react with Yes, Fyn and Slk based on sequence similarity.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Tyr527 of human Src. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or pervanadate-treated (1 mM for 15 minutes), using Non-phospho-Src (Tyr527) Antibody (upper) or v-Src antibody (lower).

Background

The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk and Hck) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain by Csk upregulates enzyme activity, whereas phosphorylation of Tyr527 in the carboxy-terminal tail renders the enzyme less active (2).

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.

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