Product Pathways - Tyrosine Kinase/ Adaptors
Non-phospho-Src (Tyr527) Antibody #2107
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R (C) | Endogenous | 60 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
C=Chicken
Species cross-reactivity is determined by Western blot.
Protocols
- 2107:
- Western Blotting
Specificity / Sensitivity
Non-phospho-Src (Tyr527) Antibody detects endogenous levels of Src only when dephosphorylated at tyrosine 527. It may also cross-react with Yes, Fyn and Slk based on sequence similarity.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Tyr527 of human Src. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from A431 cells, untreated or pervanadate-treated (1 mM for 15 minutes), using Non-phospho-Src (Tyr527) Antibody (upper) or v-Src antibody (lower).
Background
The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk and Hck) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain by Csk upregulates enzyme activity, whereas phosphorylation of Tyr527 in the carboxy-terminal tail renders the enzyme less active (2).
- Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2123 Src (32G6) Rabbit mAb
- 2101 Phospho-Src Family (Tyr416) Antibody
- 2102 Non-phospho-Src (Tyr416) (7G9) Mouse mAb
- 2105 Phospho-Src (Tyr527) Antibody
- 9935 Src Antibody Sampler Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2108 Src Antibody
- 2109 Src (36D10) Rabbit mAb
- 2110 Src (L4A1) Mouse mAb
- 2113 Phospho-Src Family (Tyr416) (100F9) Rabbit mAb
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
