Product Pathways - Tyrosine Kinase/ Adaptors
Src Antibody #2108
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-F IF-IC F | H M R Mk (C) | Endogenous | 60 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
C=Chicken
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Src Antibody detects endogenous levels of Src proteins. This antibody may cross-react with other Src family members, but does not cross-react with other non-family proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues close to the carboxy-terminus of human Src. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using Src Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Src Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Src Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using Src Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's Lymphoma, using Src Antibody preincubated with an irrelevant control peptide (left) or Src Blocking Peptide #1235 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, showing cytoplasmic and membrane localization, using Src Antibody.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using Src Antibody (blue) compared to a nonspecifc negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of MCF7 cells labeled with Src Antibody (red). Blue=DRAQ5™ fluorescent DNA dye.
Background
The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk and Hck) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain by Csk upregulates enzyme activity, whereas phosphorylation of Tyr527 in the carboxy-terminal tail renders the enzyme less active (2).
- Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2101 Phospho-Src Family (Tyr416) Antibody
- 2102 Non-phospho-Src (Tyr416) (7G9) Mouse mAb
- 2105 Phospho-Src (Tyr527) Antibody
- 2107 Non-phospho-Src (Tyr527) Antibody
- 9935 Src Antibody Sampler Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 1235 Src Blocking Peptide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
