Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

Src (36D10) Rabbit mAb #2109

Applications Reactivity MW (kDa) Source Isotype
W IP IHC-P IHC-F IF-IC F H M R Mk Pg Hm B (C) 60 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Pg=Pig  C=Chicken  Hm=Hamster  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Src (36D10) Rabbit mAb detects endogenous levels of Src proteins. The antibody may cross-react with other Src family members, but does not cross-react with other non-family proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy terminus of human Src.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Src (36D10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Src (36D10) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma, using Src (36D10) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Src (36D10) Rabbit mAb in the presence of control peptide (left) or Src Blocking Peptide #1235 (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of A-431 cells, using Src (36D10) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells labeled with Src (36D10) Rabbit mAb (red). Blue pseudocolor=DRAQ5™ (fluorescent DNA dye).


IF-F

IF-F

Confocal immunofluorescent analysis of rat colon labeled with Src (36D10) Rabbit mAb (red). Blue pseudocolor=DRAQ5™ (fluorescent DNA dye).

Background

The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk and Hck) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain by Csk upregulates enzyme activity, whereas phosphorylation of Tyr527 in the carboxy-terminal tail renders the enzyme less active (2).

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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