Product Pathways - Tyrosine Kinase/ Adaptors
Src (36D10) Rabbit mAb #2109
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP IHC-P IHC-F IF-IC F | H M R Mk Pg Hm B (C) | 60 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Pg=Pig
C=Chicken
Hm=Hamster
B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Src (36D10) Rabbit mAb detects endogenous levels of Src proteins. The antibody may cross-react with other Src family members, but does not cross-react with other non-family proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues near the carboxy terminus of human Src.
Western Blotting
Western blot analysis of extracts from various cell lines, using Src (36D10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Src (36D10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma, using Src (36D10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Src (36D10) Rabbit mAb in the presence of control peptide (left) or Src Blocking Peptide #1235 (right).
Flow Cytometry
Flow cytometric analysis of A-431 cells, using Src (36D10) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of MCF7 cells labeled with Src (36D10) Rabbit mAb (red). Blue pseudocolor=DRAQ5™ (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of rat colon labeled with Src (36D10) Rabbit mAb (red). Blue pseudocolor=DRAQ5™ (fluorescent DNA dye).
Background
The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk and Hck) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain by Csk upregulates enzyme activity, whereas phosphorylation of Tyr527 in the carboxy-terminal tail renders the enzyme less active (2).
- Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
Application References
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Companion Products
- 2101 Phospho-Src Family (Tyr416) Antibody
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- 2108 Src Antibody
- 9935 Src Antibody Sampler Kit
- 7775 Src Kinase
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 1235 Src Blocking Peptide
- 2110 Src (L4A1) Mouse mAb
- 2123 Src (32G6) Rabbit mAb
- 2113 Phospho-Src Family (Tyr416) (100F9) Rabbit mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
