Product Pathways - Tyrosine Kinase / Adaptors
Src (36D10) Rabbit mAb #2109
|2109S||100 µl (10 western blots)||---||In Stock||---|
|2109||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Hamster, Monkey, Bovine, Pig||Endogenous||60||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Chicken.
Specificity / Sensitivity
Src (36D10) Rabbit mAb detects endogenous levels of Src proteins. The antibody may cross-react with other Src family members, but does not cross-react with other non-family proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Src.
Western blot analysis of extracts from various cell lines using Src (36D10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Src (36D10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma using Src (36D10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Src (36D10) Rabbit mAb in the presence of control peptide (left) or Src Blocking Peptide #1235 (right).
Flow cytometric analysis of A-431 cells using Src (36D10) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent analysis of MCF-7 cells labeled with Src (36D10) Rabbit mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
- Zaytseva, Y.Y. et al. (2012) Cancer Res 72, 1504-17. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.