Product Pathways - Tyrosine Kinase/ Adaptors
Src (L4A1) Mouse mAb #2110
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk (C) | Endogenous | 60 | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
C=Chicken
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Src (L4A1) Mouse mAb detects endogenous levels of Src proteins. The antibody does not cross-react with other proteins.
Source / Purification
Monoclonal antibodies are produced by immunizing mice with a GST-SRC fusion protein containing the corresponding amino acid residues 1 to 110 of human Src.
Western Blotting
Western blot analysis of cell extracts from various cell lines, using Src (L4A1) Mouse mAb.
Background
The Src family of protein tyrosine kinases (including Src, Lyn, Fyn, Yes, Lck, Blk and Hck) are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain by Csk upregulates enzyme activity, whereas phosphorylation of Tyr527 in the carboxy-terminal tail renders the enzyme less active (2).
- Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2101 Phospho-Src Family (Tyr416) Antibody
- 2102 Non-phospho-Src (Tyr416) (7G9) Mouse mAb
- 2105 Phospho-Src (Tyr527) Antibody
- 2107 Non-phospho-Src (Tyr527) Antibody
- 2109 Src (36D10) Rabbit mAb
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 2113 Phospho-Src Family (Tyr416) (100F9) Rabbit mAb
- 2108 Src Antibody
- 2123 Src (32G6) Rabbit mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
