Product Pathways - Translational Control
Rictor (53A2) Rabbit mAb #2114
|W||H M R Mk||Endogenous||200||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Rictor (53A2) Rabbit mAb detects endogenous levels of total Rictor protein.
Source / Purification
Rictor (53A2) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to the sequence Gln1681 of human Rictor.
Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).
- Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.
- Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
- Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416.
- Ali, S.M. and Sabatini, D.M. (2005) J. Biol. Chem. 280, 19445-19448.
- Guertin, D.A. et al. (2009) Cancer Cell 15, 148-159. Applications: Western Blotting
- Rosner, M. et al. (2009) Hum Mol Genet 18, 3298-310. Applications: Western Blotting
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.