Product Pathways - Translational Control
Rictor (53A2) Rabbit mAb #2114
|2114S||100 µl (10 western blots)||---||In Stock||---|
|2114P||40 µl (4 western blots)||---||In Stock||---|
|2114||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||200||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Rictor (53A2) Rabbit mAb detects endogenous levels of total Rictor protein.
Source / Purification
Rictor (53A2) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to the sequence Gln1681 of human Rictor.
Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).
- Guertin, D.A. et al. (2009) Cancer Cell 15, 148-159. Applications: Western Blotting.
- Rosner, M. et al. (2009) Hum Mol Genet 18, 3298-310. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.