Product Pathways - Translational Control
Rictor (53A2) Rabbit mAb #2114
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H M R Mk | 200 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Rictor (53A2) Rabbit mAb detects endogenous levels of total Rictor protein.
Source / Purification
Rictor (53A2) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence Gln1681 of human Rictor.
Western Blotting
Western blot analysis of extracts from various cell lines, using Rictor (53A2) Rabbit mAb.
Background
Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).
- Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.
- Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
- Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416.
- Ali, S.M. and Sabatini, D.M. (2005) J. Biol. Chem. 280, 19445-19448.
Application References
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- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
